The dot blot method is to transfer the protein directly to a nitrocellulose or nylon membrane (Nitrocellulose or PVDF), then bind the target protein to a biotinylated aptamer and detect it with streptavidin-HRP/TMB (streptavidin-HRP / TMB enzyme-substrate). This method is simple, fast and inexpensive, and is suitable for academic research.
Dot blotting
Western blotting is a method of separating proteins in a sample by molecular size using polyacrylamide gel electrophoresis. The captured proteins in the gel are then transferred to a nitrocellulose or nylon membrane (Nitrocellulose or PVDF). Biotinylated specific aptamers are used to identify the bands of the target protein and are detected with streptavidin-HRP/TMB (streptavidin-HRP / TMB enzyme-substrate). It is commonly used in academic research.
Western blot
ELISA uses specific monoclonal and/or polyclonal antibodies against a specific target for capture and/or quantitative detection, and is widely used in protein research and diagnosis. In addition to maintaining the high sensitivity and specificity of ELISA, ELISA can also solve the limitations of antibodies. The selection of aptamers can be widely used in compounds with poor immunogenicity (small molecules) or toxicity.
Enzyme-linked aptamer adsorption assay (ELASA)
Competition method: Targeting small molecule targets with few binding sites, such as ractopamine, tetracycline, etc. Due to the relatively small structure of small molecule targets, the molecular surface cannot provide more than two areas for molecular probes such as antibodies or aptamers to identify and bind, so the competitive method must be used for detection. The competitive method is to first fix the target of a fixed concentration to the detection area, and fix the probe to colloidal gold. When there is no target in the sample, the probe will bind and react with the target. When the sample contains the target, the probe will first react with the target in the sample to compete for the amount of probe that will bind to the detection area. This method is used to detect small molecule targets.
Rapid Screening Test Lateral Flow - Small Molecule
Rapid Screening Test Lateral Flow - Large Molecular
Sandwich method: Targeting macromolecular targets, such as PSA, Thrombin, Virus, etc. Macromolecular targets have a relatively large structure, and the molecular surface can provide more than two areas for molecular probes such as antibodies or aptamers to identify and bind, so the Sandwich method can be used for detection. The Sandwich method uses probes that can bind to different areas of the target to form a pair. One of the probes is first fixed to the detection area, and the other probe is fixed to colloidal gold. When the sample does not contain the target, the colloidal gold has no reaction in the detection area. When the sample contains the target, the probe on the colloidal gold reacts with the target in the sample and also binds to the probe in the detection area. This method is used to detect macromolecular targets.
Semiconductor Biomedical Chip Testing Platform
The core technology used in semiconductor biomedical chip systems is "silicon nanowire field-effect transistor" (SiNW-FET), which is a semiconductor technology application that uses current signal detection and has the characteristics of fast, instant, and high sensitivity. This technology combines cross-domain technologies such as semiconductor biomedical detection chips, heterogeneous packaging, chemical surface treatment, biochemical molecular processing, microfluidic automatic control systems, micro-signal scanning measurement circuits, data processing programs, and platform mechanism design and production with electrical principles. Using Bio-FET technology as the core, a highly sensitive automated detection platform is established with a detection time of 5-30 minutes. The targets include nucleic acids, proteins or chemicals in the environment, and the high-sensitivity detection limit is 10-15 M for nucleic acids and 10^-12 g/ml for proteins. The nucleic acid probe is combined with the biomedical chip detection platform with high sensitivity and stability, and can be applied to the medical diagnosis market.
We used computer simulation software to build the 3D structure of the aptamer, and confirmed the structural stability and variability of the aptamer through software analysis.
Next, the binding sites of the aptamer and the target are simulated and predicted to screen aptamers with high affinity and specificity. Based on the computer simulation prediction results, further optimization such as sequence design and modification is performed to develop aptamers with the best affinity.
3D structure of aptamer and target binding map
Through continuous innovation and research, we are committed to pushing the boundaries of biomedicine and providing a solid scientific and technological foundation for future medical challenges.
Product Development Services
Aptamers are used in many traditional antibody-based detection and analysis platforms, showing unique advantages. Many studies have also applied aptamers to medical diagnosis, microbial testing, and food safety testing, and have found that they can solve difficulties and challenges in many fields. Applicable application platforms include Dot blot, Western blot, ELASA, Flow cytometry, In vitro/in vivo imaging, Biosensor, and Lateral flow assays.