His-Tag Protein Purification Kit
This nickel-free kit offers high-affinity, metal-free His-tagged protein capture. With ultra-low non-specific binding and zero batch variation, it preserves native activity for high-purity isolation.
This His-Tag Protein Genimer™ protein purification kit is an innovative tool developed by Chipone Biotech for the field of protein research. His-tags are the most widely used affinity tags in protein purification, but traditional nickel-ion magnetic beads (Ni-NTA) often suffer from problems such as metal ion shedding, high non-specific adsorption, and sample contamination. This product utilizes proprietary Genimer™ nucleic acid aptamer technology to provide a "metal ion-free" high-purity purification solution.
This kit is specifically designed to capture target proteins bearing the His tag. Through fully chemically synthesized nucleic acid aptamers, it achieves highly specific binding to the His-Tag. Because it does not utilize metal ion chelation, the purification process is extremely gentle, and there is no need to address metal residue issues afterward. It perfectly preserves the protein's native conformation and activity, making it an ideal choice for high-purity protein production and biophysical analysis.
Five core features of the product:
🚫 No metal ion contamination: Abandoning the traditional nickel ion chelation method, completely eliminating sample contamination caused by metal ion shedding (Leaching), without affecting downstream enzyme activity or cell experiments.
🔬 Extremely low nonspecific adsorption: Genimer™ has extremely high specificity, which can significantly reduce background protein noise interference, and obtain ultra-high purity target proteins in a single purification.
🧬 0% Batch Variation: Manufactured entirely through chemical synthesis, ensuring high consistency in affinity and loading for each batch, thus completely resolving the stability issues of biologically derived antibodies.
🌡️ Excellent chemical stability: The magnetic beads and adapters have excellent thermal stability and acid and alkali resistance, making them suitable for a variety of buffer environments and maintaining high efficiency even when operating at room temperature.
⚡ Fast and gentle purification process: Combining competitive elution technology, it can complete capture and recovery in a short time, minimizing the risk of protein denaturation in vitro.